Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A fresh pH-responsive imipenem-loaded nanocarrier against Acinetobacter baumannii with a synergetic effect

doi: 10.3389/fbioe.2023.1166790

Figure Lengend Snippet: Anti-infection effects of Imi@ZIF-8 in vivo . After successful establishment of the mouse peritonitis model in A. baumannii , BALB/c mice were intraperitoneal injected with PBS, imipenem, ZIF-8, or Imi@ZIF-8 (10 mg imi/kg). (A) Bacterial counting of liver tissues after treated with the respective materials. Data are indicated as means ± SD ( n = 3). (B) Representative images of liver tissues after H&E staining at 7 days after intraperitoneal injection of different treatments. Scale bar is 100 μm. (C) Immunohistochemistry staining of Ly6G in liver tissues at 7 days after intraperitoneal injected with PBS, imipenem, ZIF-8, or Imi@ZIF-8, respectively. (D) Immunohistochemistry staining of IL-6 in liver tissues. Scale bar is 100 μm.

Article Snippet: Anti-IL-6 and anti-Ly6G antibodies were manufactured by BOSTER Biological Technology (China).

Techniques: Infection, In Vivo, Injection, Staining, Immunohistochemistry

Journal:

Article Title: Massive Destruction of Malaria-Parasitized Red Blood Cells despite Spleen Closure

doi: 10.1128/IAI.73.10.6390-6398.2005

Figure Lengend Snippet: (A to C) Trapping capacity of the spleen during P. chabaudi infection. Splenic uptake of pRBC (A), RBC (B), and BSA-TRITC (C) was analyzed by fluorescence microscopy on the days indicated (d0 to d35) after injection of 1 × 109 PKH-26-labeled pRBC or noninfected RBC or 500 μg BSA-TRITC. (D) Semiquantitative evaluation of fluorescence intensity for 10 cryosections per mouse with three to five mice per time after injection of PKH-26-labeled pRBC (red bars), noninfected RBC (green bars), and BSA-TRITC (blue bars). Each bar indicates the mean, and each error bar indicates 0.5 standard error of the mean. The horizontal lines indicate means ± standard errors of the means for background fluorescence for cryosections of mice that did not receive an injection. (E) Numbers of F4/80+ macrophages (black bars) and Gr-1+ granulocytes (red bars) expressed as percentages of the total spleen cells. Each bar indicates the mean, and each error bar indicates 0.5 standard error of the mean. One asterisk, P < 0.05 compared with day 0 p.i.; two asterisks, P < 0.01 compared with day 0 p.i.

Article Snippet: Specifically, cells were preincubated with anti-mouse CD16/CD32 (FcIII/II receptor) F c block (BD PharMingen, Heidelberg, Germany) for 3 min and then labeled with anti-mouse F4/80 (ImmunoKontact, Wiesbaden, Germany) or anti-mouse Gr-1 (BD PharMingen) fluorescein isothiocyanate-labeled monoclonal antibodies.

Techniques: Infection, Fluorescence, Microscopy, Injection, Labeling